Background Calprotectin is a protein expressed in the cytoplasm inside the neutrophile granulocytes. During inflammatory bowel disease (IBD), the neutrophile granulocytes are involved in a complex interaction at the inflammatory area where they die and release their content into the intestinal lumen. Therefore, calprotectin in stool is a suitable marker for diagnosis and measurement of the disease-activity in patients with IBD. The most commonly used method to detect calprotectin in stool is ELISA, but the process of manual preparation of stool samples is time-consuming.Aim The objective of the study was to evaluate an extraction method that could replace manual preparation of fecal samples and to compare different methods for measuring Calprotectin in stool using two ELISA-methods from two manufacturers and one rapidtest.Methods For extraction of calprotectin from stool samples we used sample collector tubes from Epitope Diagnostics and fecal preparation kits from Roche. Two different ELISA-kits for measuring calprotectin concentration in stool were compared.

ABSTRACTBackground Calprotectin is a protein found in the cytoplasm of neutrophile granulocytes. In the course of inflammatory bowel disease (IBD), calprotectin is released during chronic inflammation in the gut. Activation of neutrophils during the inflammation is followed by activation and secretion of pro-inflammatory molecules such as calprotectin. Calprotectin is stable in stool up to 7 days and can therefore be used as a non-invasive marker for diagnosis, treatment and measurement of the disease activity in patients with IBD. The most common method for analysis of calprotectin concentration is ELISA.

Background: Inflammatory bowel disease (IBD) is an umbrella term for ulcerative colitis and Crohn's disease. These are characterized by a chronic inflammation of the intestinal mucosa. Living with chronic illness meant that life changed significantly. Quality of life was affected for these individuals and was stressful in their lives. Aim: The aim was to describe the experiences of living with inflammatory bowel disease. Method: A qualitative literature review was chosen to analyze articles that were related to the aim of this study. Results: Based on the chosen articles five themes were created; Commuting between hope and fear, symptoms of the disease and medication become everyday focus, struggling with  the new identity, desire to control the disease- do I control my illness or does it control me and social relationships are affected. Conclusion: Inflammatory bowel disease is a distressing disease and can cause limitations in social life due to lack of knowledge by others about the disease. The affected felt a loss of control in their life due to the difficulty in managing the symptoms.

n this dissertation a comparison of two evolutionary methods for evolving ANNs for robot control is made. The methods compared are SANE with enforced sub-population and delta-coding, and marker-based encoding. In an attempt to speed up evolution, marker-based encoding is extended with delta-coding. The task selected for comparison is the hunter-prey task. This task requires the robot controller to posess some form of memory as the prey can move out of sensor range.

En av de största orsakerna till den massiva förlusten av växtarter, är fragmenteringen av habitat. I Europa är öppna naturbetesmarker och ängar de habitat som genomgått de största förändringarna i och med jordbrukslandskapets omstruktureringar under 1900-talet. I den här studien inventerades kärlväxter på 5 olika öar i kulturlandskapet Lurö skärgårds naturreservat. Syftet var att ta reda på hur diversiteten bland kärlväxtarter skiljer sig mellan hävdade gräsmarker och f.d. gräsmarker där hävd upphört och hur ett förändrat landskap spelat roll för artantalets utveckling eller tillbakagång.

Causes of thrombocytopenia in dogs ? a retrospective study of 123 cases The purpose of this retrospective case study was to determine which diseases are associated with thrombocytopenia in Swedish dogs. Records of 123 dogs with thrombocytopenia were evaluated. The most common cause was neoplasia-associated thrombocytopenia (32%). The largest proportion within this group was unspecified neoplasia.

AbstractBackground: Inflammatory bowel disease involves major changes where psychological and physical stress becomes a part of ones daily life. The ability to adapt and cope well with the situation, increases the chances of feeling satisfied with life. There are various management strategies that people with IBD use to cope with their daily life.Aim: The aim of this study is to describe strategies that people with inflammatory bowel disease use to manage their daily lives. A further purpose is to describe the study group in the included articles.Method: A descriptive literature review. This study is based on 12 scientific articles, searched in the databases Cinahl and Pubmed.

Hos patient med inflammatory bowel diseases (IBD) har nutrition en central betydelse. Nutrition ska betraktas som en medicinsk behandling samtidigt som nutrition är ett av sjuksköterskans omvårdnadsansvar. Syftet var att belysa nutritionsbehovet hos patient med inflammatory bowel diseases. Resultatet baseras på 15 vetenskapliga artiklar. Resultatet visar att patient med IBD lider av malnutrition eller ligger i riskzon för att bli malnutrierad.

To conclude this year?s introductory course in Mechatronics the students, in groups of 6,constructed a prototype based on the technical requirements established by the course teachers.This year they consisted of the following: two wheels only, active balancing, remote controlledand able to stop when crossing a pre-determined marker on the ground. The project lasted for 7weeks and the result is a working prototype and this report.The report reviews the chosen solution, subsystems and the finished prototype as well assuggestions for improvements, discussion and analysis.The prototype constructed embodied most of the group?s earlier individual technical specialtiesand consists of the following: a flywheel for balancing, ultrasound for angle measurement, areflective photomicrosensor to detect the marker and Bluetooth for the hand control..

The veterinary handling of wildlife, both in zoos and in the wild, commonly requires that the patients need to be captured and immobilized for examination or treatment. This emphasises the need for quick, on-site, laboratory equipment, in order to minimize the analysis time and thus the stress for the animal and also in order to increase the possibility of starting relevant treatment early on. The aim of this pilot study was to determine whether Serum Amyloid A (SAA) could be a measured in blood samples from bottlenose dolphins (Tursiops truncatus), Asian elephants (Elephas maximus) and South American tapirs (Tapirus terrestris). SAA rises to its maximum about 24 hours after the introduction of an inflammatory agent in companion animals. This also correlates with the estimated time it takes for animals at Kolmården Wildlife Park to be examined and having blood samples collected after being observed as sick. The study also evaluated whether the Eurolyser SOLO, using a turbidometric immunoassay (TIA) developed for human diagnostics, could be used for such analysis. This pilot study was a retrospective analysis of SAA in convenience sampled frozen serum, collected from dolphins, elephants and tapirs.

The purpose of this study was to describe how adolescents aged 12-18 years with inflammatory bowel disease experience their illness and what coping strategies they use to manage their illness and improve their wellbeing. The method used was a descriptive literature study, and the result of the study included 15 scientific articles. Our results showed that IBD affected the adolescent?s everyday life and social life with friends, family and activities. The adolescents also reported that they experienced a feeling of vulnerability, altered body image and that they saw themselves as different from healthy subjects.

The purpose of this study was to investigate the kind of treatment strategies used by Swedish Veterinarians with cattle practice when treating calves with symptoms of pneumonia. A questionnaire, consisting of seven questions, was sent to one hundred veterinarians who have had practice with cattle and are employed by the Swedish Board of Agriculture. The survey was answered by a total of 73% of the Veterinarians. Of the respondents, 73 % use Penicillins as their first choice of drug for treating calves with symptoms of pneumonia, and as their second choice 64 % use Tetracycline. Supplementary treatment such as NSAID (non-steroidal anti-inflammatory drugs) and/or SAID (steroid anti-inflammatory drugs) are used by 97.5 % of the respondents.

Background: Inflammatory bowel disease (IBD) includes Crohn´s disease (CD) and ulcerative colitis (UK). The diseases are chronic and have a pattern of relapses interspersed with relatively symptom-free periods. Common symptoms during relapse are diarrhea, abdominal pain and weight loss. Since you have IBD for life it is important to find ways to relate to and cope with the disease. In that process a nurse can be of great help.Aim: The purpose of this literature review was to increase the understanding of how adult patients with inflammatory bowel disease experience their daily lives.  Method: The method used was a literature review.

An inflammatory reaction is induced after release of proinflammatory mediators such asinterleukin 1 and 6 and tumour necrosis factor ?. These mediators stimulate the liver tosuppress the syntheses of albumin and endure the syntheses of acute phase protein forinstance C-reactive protein. The aim of this paper was to perform a method validation on animmune turbidimetric assay to quantify C-reactive protein in canine serum at the laboratory atSkara Animals Hospital, Skara, Sweden. The validation involved evaluation of the assaylinearity, precision, stability and recovery.The method was proved to be linear for both TruLab control and Medinor control.

Hyperthyroidism and chronic kidney failure (CKD) are common diseases in older cats. It can be difficult to evaluate kidney function in cats suffering from both conditions simultaneously. In the daily clinic serum creatinine is the analysis of choice when evaluating kidney function and secondary glomerular filtration rate (GFR). This marker does not, however, detect kidney damage until about 75 % of the function is lost. There are several other techniques available when investigating GFR but none are readily available for the small clinic.